Table 1.
Optogenetic agents and tests reported in this study
| Viral Vector | Promoter, Opsin, and Fluorescent Marker | Source and Preparation | Opsin Action | Purpose in This Study (Monkey - Experiment) |
|---|---|---|---|---|
| Lenti | CaMKIIα-ChR2-mCherry | D, C, S | Depolarize | M - Optical imaging |
| Lenti | CaMKIIα-ArchT-GFP | B, C, S | Hyperpolarize | M - Histology |
| C - In vivo epifluorescence | ||||
| Lenti | CaMKIIα-ChR2-EYFP | D, C, S | Depolarize | C - In vivo epifluorescence |
| C - Electrophysiology | ||||
| AAV5 | CaMKIIα-C1V1 (E122T/E162T)-EYFP | D | Depolarize | A - Electrophysiology |
| A - In vivo epifluorescence | ||||
| Lenti | CaMKIIα-C1V1 (E162T)-EYFP | D, C | Depolarize | A - Electrophysiology |
| A - In vivo epifluorescence |
Lenti, lentivirus; AAV5, adeno-associated virus serotype 5; CaMKIIα, α-calcium/calmodulin-dependent protein kinase II; ChR2, channelrhodopsin-2; mCherry, a red fluorescent protein; ArchT, archaerhodopsin-T inhibitory opsin; GFP, green fluorescent protein; EYFP, enhanced yellow fluorescent protein; C1V1, ChR2 variant; B, Boyden laboratory, Massachusetts Institute of Technology; C, A. Cetin and E. M. Callaway, Salk Institute; D, Deisseroth laboratory, Stanford University; S, Salk Institute viral vector core.