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Involvement of NOX activity in BLM-induced 8-epi-PGF2α release. Levels of F2-isoprostanes (8-epi-PGF2α) were determined in the media of HLF from 3 donors treated with BLM (50 mU/mL) for 24 h, in presence or absence of DPI (1 μM), a broad NOX activity inhibitor. Data are mean ± SD of 3 separate experiments, each performed in triplicate. *P < 0.05 versus control (C); **P < 0.01 versus control (C); ## P < 0.01 versus BLM.
