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. 2013 Aug 16;3(8):e132. doi: 10.1038/bcj.2013.30

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Copyright © 2013 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Additive anti-ATL cell effects by the combined dosage of 17-DMAG and Nutlin-3a. (a) ATL cell lines C8166, MT4, ED and ATL4 were treated with either suboptimal single dose of 17-DMAG (0.1 μM, black circles) and Nutlin-3a (1 μM, white circles)⁴¹ or both (white triangles) for 3 days and harvested for caspase-3/7 assays (a) or CCK-8 assays (b) as described in Figure 2. Each untreated cell's value was set as 1. (c) Each caspase-3/7 (apoptotic) value was divided by CCK-8 (growth arrest) value to manifest the additive effects (see the Discussion section). CCK-8, Cell Counting Kit 8.