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. 2011 Oct 19;3(4):3909–3920. doi: 10.3390/cancers3043909

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — ZNF143 expression under serum condition (a) PC3 mock cells and CL5 cells with forced expression of ZNF143 were cultured under serum starvation for 12 h and 24 h, respectively. And re-seeding with medium containing serum was performed. Each nuclear protein (100 μg) was used for western blotting. BAF57 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1) was used as internal control. Lane 1 and 4; pre-serum starvation, lane 2; serum positive for 12 h, lane 3; serum free for 12 h, lane 5; serum positive for 24 h, lane 6; serum free for 24 h; (b) Cells under serum starvation were re-seeded with medium containing serum and collected at indicated time. Each nuclear protein (100 μg) was used for western blotting. BAF57 was used as internal control. Lane 1 and 3; pre-re-seeding, lane 2 and 4; 1 h and 2 h after re-seeding, respectively.

Figure 3. — ZNF143 expression under serum condition (a) PC3 mock cells and CL5 cells with forced expression of ZNF143 were cultured under serum starvation for 12 h and 24 h, respectively. And re-seeding with medium containing serum was performed. Each nuclear protein (100 μg) was used for western blotting. BAF57 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1) was used as internal control. Lane 1 and 4; pre-serum starvation, lane 2; serum positive for 12 h, lane 3; serum free for 12 h, lane 5; serum positive for 24 h, lane 6; serum free for 24 h; (b) Cells under serum starvation were re-seeded with medium containing serum and collected at indicated time. Each nuclear protein (100 μg) was used for western blotting. BAF57 was used as internal control. Lane 1 and 3; pre-re-seeding, lane 2 and 4; 1 h and 2 h after re-seeding, respectively.