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Serum IgM antibodies and GS-I binding to 4T1 cell lysate. Whole-cell lysate from 4T1 cell line was prepared. Mice (5/group) were pre-bled and immunized with peptide 107 on days 0 and 14. Sera were collected 7 days after the peptide boost and pooled for each group. Western blot analysis: Sera were diluted 1:1000 for the western. Anti mouse IgM used as secondary antibody. Binding with biotin-conjugated GS-I was followed by streptavidin-HRP.
