Table 1.
Summary of DNA sequencing platforms 1.
| Platform | Technology | Model | Length of read | Throughput (per day) | Company |
|---|---|---|---|---|---|
| Automated Sanger sequencer | Capillary electrophoresis, BigDye®-terminator chemistry | 3730 xl | Up to 900 bp | <3 Mb | Applied Biosystems |
| 454 | Pyrosequencing | GS FLX+ | 500–700 bp | 700 Mb | 454 Life Sciences (Roche) |
| SOLiD™ | Sequencing by ligation | 5500 xl | 75 bp | 30 Gb | Life Technologies (ABI) |
| Illumina | Clonal single molecule array | HiSeq2000 | 50–150 bp | Up to 55 Gb | Illumina, Inc. |
| Complete genomics | DNA nanoball array, ligation-based sequencing | N/A 2 | 70 bp | 8.8 Gb | Complete Genomics |
| Ion Torrent | Hydrogen ion semiconductor | Ion 316 Chip | 100 bp | 100 Mb 3 | Life Technologies (ABI) |
| HeliScope™ | Imaging single nucleotide incorporation | Single Molecule Sequencer | 35 bp | 1 Gb | Helicos |
| PacBio | SMRT™ technology | PacBio RS | >1,000 bp | 500 Mb 4 | Pacific Biosciences |
1
Typical performances of selected model systems are listed based on the marketing materials from each company. Actual results may vary depending on specific sample and genomic characteristics;
2
Not commercially available. Complete Genomics offers in-house sequencing services bundled with web-based data analysis;
3
Output per chip;
4
Based on Enterobacteria phage λ at 45 Mb/SMRT cell, 12 SMRT cells/day.