Figure 2.

Depletion of Wip1 enhances IR-induced apoptotic cell death. (a) HeLa or LNCaP cells were irradiated with 10 Gy. After 72 h, the cells were harvested and stained with propidium iodide (PI) and annexin V-fluorescein isothiocyanate (FITC). The proportions of apoptotic cells within the annexin V-FITC and PI-positive subpopulations were determined using fluorescence-activated cell sorting (FACS). (b) LNCaP cells were transfected with 50 nM of either control small interfering (si)RNA or Wip1 siRNA. At 48 h post-transfection, the cells were irradiated with 10 Gy. After 72 h, the cells were stained with PI and annexin V-FITC. Apoptotic cell death was determined by FACS. (c) LNCaP cells were transfected with 50 nM of either control siRNA or Wip1 siRNA for 36 h, and then irradiated with 10 Gy. After 40 h, the transfectants were assayed for caspase-3 activity. All assays were performed in triplicate. The data show the mean±S.D. (*P<0.05)