Figure 3.

Differential regulation of STAT3/CCL2 pathway by Casodex, MDV3100, and ASC-J9. (a) Casodex and MDV3100 enhanced, but ASC-J9 suppressed CCL2 expression. The C4-2B and TRAMP-C1 cells were treated with 10 μM Casodex (CASO), 10 μM MDV3100 (MDV), 5 μM ASC-J9 (ASC), or vehicle control (NC), incubated in 10% CD-FBS RPMI media at 10 nM DHT concentration for 24 h, and the gene expression was determined by qPCR. (b) Casodex and MDV3100 enhanced, but ASC-J9 suppressed, STAT3 activation. The effects of three different anti-A/AR compounds on the expression of CCL2, PIAS3, pSTAT3, STAT3, and GAPDH in C4-2B and TRAMP-C1 cells were analyzed by western blotting. (c) ASC-J9 inhibited STAT3/CCL2 signaling via AR-independent pathway. The effects of three different anti-A/AR compounds on the expression of CCL2, PIAS3, pSTAT3, STAT3, and GAPDH in AR-negative PC3 cells were analyzed by western blotting. (d) Illustration of AR-dependent and AR-independent pathway of STAT3/CCL2 regulation by ASC-J9. Error bars=mean±S.E.M., *P<0.05, **P<0.01, ***P<0.001. N.S., not significant