Figure 1.

The expression pattern of Gal-3 and AR in human prostate cancer cells. The expression levels of related proteins were analyzed by western blot. (a) The expression of Gal-3 and AR in LNCaP, DU145, and PC3 cells. (b) Gal-3 modulation had no effect on endogenous AR level. VC, cells transfected with a non-target control vector; no. 29-11 and no. 29-23, LNCaP clones with Gal-3 overexpression; siGal3-11 and siGal3-19, PC3 clones with Gal-3 knockdown. (c) AR overexpression had no effect on Gal-3 level in PC3 cells. PC3-AR, PC3 cells transfected with AR. β-Actin was used as the loading control. Data are representative of three independent experiments