Figure 1.

bDMC triggers irreversible cell death in HCT116 cells. (a) Cytometric cell-cycle analysis of HCT116 cells treated with 30 μM bDMC for 4, 8, 16, 24 h and released in drug-free medium for additional 16 and 24 h (16hR and 24hR) following 24 h of bDMC treatment. (b) Percentages of SubG1 events detected by PI cytometric analysis following the indicated time exposure to bDMC. Reported values are an average of 10 independent experiments±S.D. (c) The percentage of Annexin V-positive cells after 24 h treatment with 30 μM bDMC is compared with Control (CTR) and DMSO-treated cells. Data are means of three independent experiments±S.D. (d) Western blot expression analysis of γH2AX and cleaved PARP1 following time-dependent administration of 30-μM bDMC to HCT116 cells. Actin was used as loading control