Figure 6.

bDMC affects NF-Y subunits expression and sub-cellular localization. (a) Western blot analysis of total extracts of DMSO and bDMC (30 μM)-treated cells (24 h) with antibodies against the three NF-Y subunits. (b) Semi-quantitative RT-PCRs of NF-YA, NF-YB and NF-YC subunits following 24 h treatment with DMSO and 30 μM bDMC. (c) Expression levels of NF-YB and NF-YC in nuclear and cytosolic fractions of HCT116 cells treated with 30 μM bDMC for 24 h. Tubulin and H3 were used as loading control for cytosolic and nuclear extracts, respectively. (d) Time course analysis of NF-Y subunits expression in nuclear and cytoplasmic cellular compartments. (e) Nuclear expression levels of NF-YB, NF-YC and p53 following co-incubation of 30 μM bDMC with LMB for 8 h versus bDMC alone. Histone H3 was used as loading control. (f) Expression levels of SP1 in total and chromatin-enriched extracts following time course incubation with 30 μM bDMC