Table 1. RNAi screen parameters from different published influenza RNAi screens.

	Tran et al. (this study)	Shapiraet al.11	Koniget al.14	Karlaset al.13	Brasset al.17	Suiet al.15	Haoet al.16
Approach	RNAi screen	Yeast-2-hybrid• RNAi screen	RNAi screen	RNAi screen	RNAi screen	Random homozygous gene perturbation	RNAi screen
Screen parameter	Selection of surviving cells after influenza infection	Level of Renilla luciferase expression in 293T reporter cells	Level of Renilla luciferase expression	Level of Renilla luciferase expression in 293T reporter cells	Stained for surface expression of HA	Host gene perturbation	Level of reporter gene expression
End point (h.p.i.)	72 h.p.i.	48 h.p.i.	36 h.p.i.	24 h.p.i.	12 h.p.i.	48 h.p.i.	24–48 h.p.i.
No. of genes screened	21,415	1745	19 628	22 843	17 877	NA	13 071
Delivery method	Lentiviral	Transfection	Transfection	Transfection	Transfection	Lentiviral	Infection with recombinant virus
Total candidates	1256 Genes	616 genes	295 Genes	287 Genes	312 Genes	110 Clones	110
Genes in biological replicates	138	NA	NA	NA	NA	NA	NA
Virus used	NY55 (H3N2), PR8 (H3N2), pandemic California (H1N1)	PR8 (H1N1), Udorn (H3N2)	Recombinant WSN (H1N1), HA-luciferase	WSN (H1N1)	PR8 (H1N1)	Udorn (H3N2)	Recombinant WSN (H1N1) with VSV G protein and NA-luciferase
Cell line used in screen	A549 cells	293T and HBEC cells	A549 cells	A549 and 293T cells	U2OS cells	MDCK cells	Drosophila cells

NA, not available