Figure 2.

A–H, Stresscopin colocalization and regulation by stress. Combined stresscopin in situ hybridization and immunohistochemistry for a marker of neurons (NeuN) or glia was conducted on rat retrosplenial (A, D) and PFC (B, E, G) or human PFC (C, F, H). Black emulsion grains can be clearly seen over brown DAB-stained NeuN-positive cells (arrow). I, J, The influence of chronic stress, either restraint or unpredictable stress (I), or antidepressant administration (3 weeks) on stresscopin expression (J) was determined using a ³⁵S-labeled antisense riboprobe. The results are expressed as the mean ± SEM (n = 5 for stress, n = 4 for antidepressants).