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. Author manuscript; available in PMC: 2013 Sep 5.
Published in final edited form as: Cancer Res. 2010 Apr 27;70(10):3890–3895. doi: 10.1158/0008-5472.CAN-10-0155

Figure 2.

Figure 2

HSV ICP6-negative mutants are responsive to the amplifying-activities of DP and DL. (a) Effects of DP (red bars) and DL (blue bars) on wild-type HSV-1 or oHSV's carrying deletion-mutations present in G47Δ. Values are the average ± SEM and represent three independent experiments. (b) Quantitative RT-PCR analysis of ICP4, UL23 or UL44 viral transcript levels in PC3 cells untreated or treated with DP or DL and infected with G47Δ (left panel), F△6 (middle panel) or strain F (right panel) for 24 or 48 hrs. GAPDH was used to normalize for mRNA levels. (c) Pharmacological inhibitors of PDE, PK or ENT1 and nucleosides (ns) were assessed for their ability to promote increased G47Δ replication by plaque assays. (d) Ribonucleotide reductase activity is transiently elevated by DP and DL in PC3 (left panel) but not PANC-1 (right panel) cells. Results are expressed as a percentage of the control value (relative RR activity).