Table 2.
PEG 600 %(v/v) | S20,w (Svedbergs) | Frictional ratio | MW ± SEa |
---|---|---|---|
0 | 1.99 | 1.013 | 7347 ± 77 |
10 | 1.99 | 1.013 | 7110 ± 79 |
20 | 1.93 | 1.044 | 7269 ± 188 |
30 | 1.69 | 1.192 | 7214 ± 220 |
aThe sedimentation coefficients were determined by extrapolation to zero concentration using five concentrations of hTel22 in the range of 2.0–7.6 μM. The true MW assuming 2 K+ bound of 7045 was used for the calculation of frictional ratios assuming 0.3 g/g water bound. MW ± SE was determined from the analysis of five concentrations of hTel22 for each concentrations of PEG using Sedfit software.