Figure 1.

Predicted structures of Tlc1 RNA variants. (A) Schematic for the predicted overall secondary structure of the budding yeast telomerase RNA Tlc1. Important features such as the Sm-binding site near the 3′-end, the template (in red) the pseudo-knot and stem IVc (dashed oval) are highlighted. Colored elements on stem IVc are the CS2 element (in blue), the CS2a element (in green) and the TeSS (in orange). (B) MFold predicted secondary structures of the stem IVc arm of the Tlc1 RNA and key subelements. Blue: CS2 element. Green: CS2a element as in (A). Stars indicate co-varying base pairs. Red: positions of the mutated nucleotides in the different constructs.