Skip to main content
. 2013 Jun 19;41(16):7861–7874. doi: 10.1093/nar/gkt543

Figure 2.

Figure 2.

The HCV 3′UTR exhibits strong, specific and conserved interaction with the 40S subunit. (A). Binding isotherms for the 3′UTR-40S interaction. The HCV 3′UTR interacts with the 40S subunits isolated from both HeLa extract and RRL, whereas an RNA of similar size, corresponding to part of the HCV NS5B protein-coding region directly upstream of the 3′UTR (nucleotides 9184–9386 from HCV strain H77) showed no obvious interaction. (B). The 3′UTR-40S interaction is subject to competition by unlabeled 3′UTR competitor (left panel). The 3′UTR-40S interaction is not subject to competition by a random 40-nt mRNA mimicker (right panel). The amount of competitor used is indicated. The bound fraction shown has been normalized. (C). Binding isotherm of the HCV 3′UTR from different genotypes binding to the 40S subunit. The Kd values are 6.7 ± 0.7 nM (genotype 1a), 4.7 ± 0.9 nM (genotype 1b), 3.5 ± 1 nM (genotype 2a), 3.7 ± 0.4 nM (genotype 3a), 6.7 ± 0.7 nM (genotype 6b).