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. 2013 Jun 26;41(16):7673–7682. doi: 10.1093/nar/gkt562

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Depletion of TbRAP1 resulted in derepression of BES-linked VSGs in PF cells (A) and derepression of mVSGs in PF (B) and BF (C) cells. Steady–state mRNA levels for BES-linked VSGs 2, 3, 6, 11, mVSGs 397, 531, 639, 653 and control genes at days 0, 2 and 3 (PF) or at 0, 24 and 36 h (BF) after adding doxycycline in TbRAP1 RNAi cells were estimated using qRT-PCR and normalized against that before induction. Day 0 value is set to 1 but not shown. The fold change in mRNA levels for three independent induction experiments was shown.