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. 2013 Jun 25;41(16):7905–7919. doi: 10.1093/nar/gkt565

Figure 1.

Figure 1.

miR-195 associates with the stim1 mRNA. (A) Schematic representation of the stim1 mRNA depicting target site for miR-195 in its 3′-UTR. Alignment of the stim1 mRNA sequence with miR-195: top strand, stim1 mRNA; bottom strand, miR-195. (B) Levels of biotinylated miR-195 after transfection for 24 h: (a) schematic representation of biotinylated miR-195 and scramble oligomer; (b) miR-195 levels as measured by Q-PCR analysis; and (c) U6 RNA levels. Values are the means ± SEM from three separate experiments. *P < 0.05 compared with cells transfected with control scramble oligomer. (C) Binding of biotinylated miR-195 to mRNAs encoding Stim1, TRPC1, Cav-1 and c-Myc: (a) levels of mRNA in the materials pulled down by biotin-miR-195; and (b) levels of total input mRNAs. *P < 0.05 compared with cells transfected with control scramble oligomer. (D) Changes in the levels of miR-195–stim1 mRNA association after addition of fraction of stim1 mRNA sense strand to the reaction medium: (a) schematic representation of stim1 mRNA sense strand (RSS); (b) levels of mRNA in the materials pulled down by biotin-miR-195; and (c) levels of total input mRNAs. *P < 0.01 compared with cells transfected with scrambled oligomer.