Figure 4.

Inhibition of subgenomic HCV replicon replication in Huh-7 cells. Huh-7 cells that permit the autonomous replication of subgenomic HCV replicons, were transfected with 3 μg of the different aptamers (independently). Viral RNA was isolated and quantified as described in Experimental Section. The bar chart shows the (+) strand HCV RNA levels normalized to the value obtained with the control RNA, an 80 nt-long unrelated RNA used as an internal control. (−) RNA, cells treated with TransFectin^TM in the absence of any RNA. Values are the mean of at least 4 independent experiments.