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Cell numbers from FACS analysis of AAV subretinal transduction with increased levels of MFG-E8. The number of cells that are transduced (GFP +ve) or not (GFP –ve) by virus expressing eGFP are shown.
| Population | # Events | % Total | # Events | % Total |
|---|---|---|---|---|
| Retina RAD | RPE RAD | |||
| Live singlet cells | 4,297 | 100 | 864 | 100 |
| GFP −ve | 4,035 | 93.9 | 562 | 65 |
| GFP +ve | 215 | 5 | 276 | 31.9 |
| Retina RGD | RPE RGD | |||
| Live singlet cells | 3,743 | 100 | 314 | 100 |
| GFP −ve | 3,219 | 86 | 298 | 95 |
| GFP +ve | 429 | 11 | 8 | 5 |
