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. Author manuscript; available in PMC: 2013 Sep 5.
Published in final edited form as: Nat Protoc. 2013 Feb;8(2):418–429. doi: 10.1038/nprot.2013.015

Fig. 3. AP-fusion protein binding to the mouse embryo hindbrain.

Fig. 3

Freshly dissected E12.5 hindbrains were reacted with the AP-tagged VEGF165 (a,b,d,e) and SEMA3A (c,f). (a-c) Flatmounting the hindbrain with the pial side up allows visualisation of radial vessels entering the brain (arrowhead); note that VEGF165 also detects axon tracts in the dorsolateral hindbrain (arrow). (d-f) Flatmounting the hindbrain with the ventricular side up allows visualisation of the subventricular vascular plexus. The dotted boxes in (a,d) indicate the areas shown at higher magnification in (b,e). Scale bars: (a,d) 500 μm, (b,c,e,f) 100 μm. All animal procedures were performed in accordance with institutional and UK Home Office guidelines.