Table 3. Examples of embryonic ages suitable to quantify various angiogenic parameters.

side	age	angiogenic parameter	antibody	magnification
ventricular	E10.5-11.5	tip cell and filopodia number	IB4 (IF)	40×, 63×
	E10.5-12.5	branchpoints or intersections	IB4, PECAM (IF or HRP)	10×
	E10.5-12.5	vessel diameter	IB4, PECAM (IF)	63×
	E10.5-12.5	endothelial cell proliferation	pH3 + IB4 (IF)	10×
	E10.5-12.5	tissue macrophage number	F4/80 ± IB4 (IF)	10×, 20×
	E10.5-12.5	pericyte density	NG2 + IB4 (IF)	10×, 20×
pial	E11.5-12.5	density of radial vessels	IB4, PECAM (IF or HRP)	10×
	E10.5-12.5	vessel diameter	IB4, PECAM (IF)	63×
	E10.5-12.5	endothelial cell proliferation	pH3 + IB4 (IF)	10×