Figure 2.

Generation of lentivector-delivered shRNA for specific knockdown of VEGFA in Müller cells. A: Diagram of the pFmCD44.1GW lentivector containing the glia-specific CD44 promoter driving an miR-30–based shRNA cassette and an RFP marker. B: HEK293 reporter cell line expressed GFP-tagged rat VEGF₁₂₀ or VEGF₁₆₄. C: HEK reporter cell lines to VEGF₁₂₀ and VEGF₁₆₄ were transfected with RFP-expressed lentivector VEGFA-shRNA plasmids, and GFP fluorescence (infectivity) was measured by flow cytometry. D: Quantification of percentage silencing of VEGF₁₂₀ and VEGF₁₆₄ by VEGFA-shRNAs from fluorescence-activated cell sorter analysis (n = 3). Results are means ± SEM.