Skip to main content
. 2013 Sep;183(3):964–974. doi: 10.1016/j.ajpath.2013.05.011

Figure 4.

Figure 4

In vivo analysis of lentivector-delivered shRNA transduction in retina of pups raised in the rat 50/10 OIR model at p18 after subretinal injection at p8. A: Images by Micron III retinal imaging microscope showed GFP expression in retina of pups after lentivirus injection compared with the PBS-injected pup. B: GFP expression is localized with CRALBP-labeled Müller cells in retinal cryosections at p18 taken from pup eye injected at p8 with lentivirus containing CD44 promoter driving GFP expression. C: ELISA of retinal VEGFA protein at p18. ∗∗∗P < 0.001 versus uninjected room air raised (RA), ††P < 0.01 versus PBS, and ‡P < 0.05 versus luciferase-shRNA. Results are means ± SEM (n = 6 to 8). D: IHC of VEGFA protein in glutamine-synthetase–labeled Müller cells at p18, showing reduced immunoreactivity of VEGFA in VEGFA-shRNA–treated eyes compared with uninjected or control injected eyes from the 50/10 OIR model at p18. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; RPE, retinal pigment epithelium.