Figure 1.

Insulin treatment exacerbates retinal vascular permeability and induces increased expression of ADAM10 and cleaved Btc in the retina. A: Blood glucose levels in normal (Ctrl), insulin-treated (Ins), STZ-induced diabetic (DM), and STZ-induced diabetic, insulin-treated mice (DM+Ins). B: Leakage of Evans Blue dye from mouse retinal vessels in Ctrl, DM, Ins, and DM+Ins mice was quantitated in whole-mount retinas 1 hour after perfusion with the dye. C: Representative Western blots of soluble Btc, ADAM10, and actin in retinas from Ctrl, DM, and DM+Ins mice. Data are expressed as means ± SEM. n ≥ 5 mice per group. ^∗∗∗P = 0.001, ^†P < 0.0005 analysis of variance.