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. 2013 Sep;183(3):987–995. doi: 10.1016/j.ajpath.2013.05.017

Figure 3.

Figure 3

Inhibition of Btc can prevent insulin-mediated disruption of tight junctions in retinal pigment epithelial cells. A: Representative immunoblots of soluble Btc, ADAM10, HIF-1α, and actin in ARPE-19 human RPE cells exposed to increasing concentrations of insulin. B: Immunofluorescent ZO-1 expression in ARPE-19 cells exposed to insulin in the presence or absence of ADAM10 siRNA or Btc siRNA. Arrowheads indicate the disruption of ZO-1. C: Representative immunoblots of soluble Btc and ADAM10 in control ARPE-19 cells treated with Btc siRNA or ADAM10 siRNA. D: Representative immunoblots of soluble Btc, ADAM10, EGFR, phosphorylated EGFR (pEGFR), and actin in ARPE-19 human RPE cells exposed to insulin (100 nmol/L) in the presence of Btc siRNA or ADAM10 siRNA, with quantitation of the EGFR/pEGFR ratio after no treatment or treatment with Btc siRNA or ADAM10 siRNA. ∗P < 0.05. Data are expressed as means ± SEM. n ≥ 5 mice per group. NT, non-targeting control RNA.