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. 2013 Sep;183(3):831–840. doi: 10.1016/j.ajpath.2013.05.010

Figure 2.

Figure 2

TIMP3 is essential for ITGA7-mediated suppression of cell growth. A: Immunoblot analysis of ITGA7, TIMP3, and β-actin. PITT1 or PITT2 cells (pCDNA4-ITGA7/pCDNA6TO transfected PC3 clones) were induced with or without 5 μg/mL tetracycline (Tet) and treated with siRNA specific for TIMP3 (siTim) or scrambled control (Scr) for 24 hours. The protein extracts were separated by SDS-PAGE and immunoblotted with antibodies specific to ITGA7, TIMP3, or β-actin. B: Colony formation assays of replica samples of A. Triplicate experiments were performed on each assay. ∗∗P < 0.01 (t-test). C: BrdU labeling and cell cycle analyses of replica samples of A. Triplicate experiments were performed on each assay. ∗∗∗P < 0.001 (t-test).