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. 2013 Sep;183(3):831–840. doi: 10.1016/j.ajpath.2013.05.010

Figure 3.

Figure 3

Induction of ITGA7 expression decreases TNF-α level and reverses nuclear localization p65 NF-κB subunit. Lanes 1–4: Immunoblots of ITGA7, TNF-α, cyclin D1, and β-actin from PITT1 and PITT2 cells induced with or without tetracycline (Tet). Lanes 5–12: Chromatin association of p65 NF-κB subunit. PITT1 and PITT2 cells were fractionated into chromatin (Chr) and non-chromatin (NC) fractions. Immunoblots were generated using antibodies specific for p65, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), or histone 3.