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. 2013 Sep;183(3):831–840. doi: 10.1016/j.ajpath.2013.05.010

Figure 4.

Figure 4

TIMP3 is required for ITGA7-induced cyclin D1 down-regulation. PITT1 and PITT2 cells were induced with or without tetracycline (Tet) and treated with siRNA specific for TIMP3 (siTim) or scrambled (Scr) controls for 24 hours. Lanes 1 to 8: Immunoblots were generated from the protein extracts using antibodies specific for ITGA7, TIMP3, TNF-α, cyclin D1, or β-actin. Lanes 9 to 24: Chromatin association of p65 NF-κB subunit. PITT1 and PITT2 cells were fractionated into chromatin (Chr) and non-chromatin (NC) fractions. Immunoblots were generated using antibodies specific for p65, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), or histone 3.