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. 2013 Sep;183(3):831–840. doi: 10.1016/j.ajpath.2013.05.010

Figure 6.

Figure 6

TIMP3 binding motif peptide interferes with cell growth suppression by ITGA7. A: Immunoblot analysis of ITGA7, TBM-FLAG, and β-actin. PITT1 and PITT2 cells were transfected with pCMV-TBM-FLAG (TIMP3 binding motif-FLAG) or vector control (V). These cells were treated with or without tetracycline (Tet). Immunoblot assays were performed using antibodies specific for ITGA7, FLAG, or β-actin. B: Colony formation assays of duplicate experiments of A. Triplicate experiments were performed on each assay. C: BrDU labeling and cell cycle analysis of duplicate experiments of A. Triplicate experiments were performed on each assay. ∗∗P < 0.01 (t-test).