Skip to main content
. 2013 Sep;183(3):831–840. doi: 10.1016/j.ajpath.2013.05.010

Figure 7.

Figure 7

Laminin inhibits ITGA7-TIMP3 signaling and growth suppression. A: Knocking down of β1-laminin enhanced ITGA7-TIMP3 signaling. Lanes 1 to 8: Immunoblots were generated from the protein extracts from PITT1 and C5 cells induced with or without tetracycline (Tet) and treated with siRNA specific for β1-laminin (siLam) or scrambled control (Scr), using antibodies specific for ITGA7, β1-laminin, cyclin D1, TNF-α, or β-actin. Lanes 9 to 24: Chromatin association of p65 NF-κB subunit. PITT1 and C5 cells were fractionated into chromatin (Chr) and non-chromatin (NC) fractions. Immunoblots were generated using antibodies specific for p65, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and histone 3. B: Colony formation assays of replica samples of A. C: BrdU labeling and cell cycle analyses of replica samples of A. ∗∗P < 0.001.