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Wdfy1 mRNA expression in PSCs isolated from B6N and B6J substrains. Wdfy1 mRNA expression was normalized to Rplp0 and expressed as fold change over the level in PSCs isolated from B6N mice. Data are expressed as means (n = 3) with 95% confidence intervals. Confidence intervals were calculated using the ΔCT values before exponential transformation to fold increase in mRNA and are therefore asymmetric about the means. ∗P < 0.05.
