Figure 2.

Collagen content in the pancreas assessed by Sirius Red staining after induction of CP by repetitive cerulein injections. A: Representative pancreatic sections of pancreas from saline-treated control B6N and B6J mice and from cerulein-treated B6N and B6J mice. Control mice exhibit some perilobular collagen, with no difference between substrains. Cerulein-treated mice demonstrate extensive interlobular and periacinar collagen staining, indicating robust fibrogenesis in this model of CP. Collagen staining was more pronounced in B6J than in B6N mice. B: Quantification by morphometric analysis showed that the relative amount of pancreatic collagen was greater in B6J than in B6N mice. Data are expressed as means ± SEM. n = 6. ^∗P = 0.0005. Original magnification, ×200.