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B6J mice are nicotinamide nucleotide transhydrogenase (NNT) deficient. A: PCR analysis of genomic DNA from B6J and B6N mice confirmed that B6J mice lack the Nnt gene. B: Western blotting of pancreatic extracts demonstrated the presence of NNT protein in B6N and its absence in B6J mice. β-Actin was used as in internal control.
