Figure 1.

Cell proliferation induced by OPG in mixtures of LAM cells (A), pulmonary artery smooth muscle cells (B), and normal human fibroblasts (C). A total of 2000 cells per well were plated in 96-well plates and incubated overnight in serum-free medium with 10, 50, or 100 ng/mL of OPG, RANKL, TRAIL, or complete medium (CM), and cell growth was measured using a colorimetric assay that produced a compound that was directly proportional to the number of living cells. Experiments were performed three times. Data are reported as a percentage of the appropriate vehicle-containing control (V), and the LAM data are the average of results from four cell lines. P values for treatment versus control are above bars. Data are means ± SEM. ***P < 0.001.