Figure 3.

TSC2 LOH in cells that migrated in response to OPG. Cells were stimulated overnight with medium without serum (B) or medium with 10% serum (C) or 100 ng/mL of OPG (D) or CCL2 (E) in the lower chamber of a Boyden chamber. DNA was prepared from cells that migrated, and PCR was performed to quantify chromosome 16 marker D16S3395. Q^LOH values were calculated relative to peak heights in samples amplified from the blood (A, control) of the patient who was the source of the heterogeneous mixtures of cells grown from LAM lung. A sample with a Q^LOH score of <0.6 was categorized as having LOH. Experiments were performed with two different heterogeneous mixtures of cells grown from LAM lungs (from two different patients) at least two times each. OPG and CCL2 each selectively stimulated migration of cells with TSC2 LOH. AU, arbitrary unit.