Figure 6.

Depletion of RAMP1 reduces tumorigenicity and proliferation in vivo. A–C: Xenografts of shRAMP1 1 PC3 cells grown s.c. in nude mice show reduced tumor growth (A) and final tumor weight (B) and size (C). D: Representative images of H&E-stained sections of tumor grafts. E: Ki-67 proliferation index. F: pHH3, phosphorylated-histone H3 serine 10 index. G: Apoptotic index by activated caspase-3 staining. The total number of cells staining positive for either Ki-67, pHH3, or activated caspase-3 in five random high-power field (HPFs) were counted for each graft section. Data are reported as means ± SEM. ^∗P < 0.05.