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. 2013 Sep;183(3):951–963. doi: 10.1016/j.ajpath.2013.05.021

Figure 7.

Figure 7

Down-regulation of RAMP1-altered gene expression and MAPK signaling in PC-3 cells. A: GSEA shows a list of selected gene sets enriched in shControl relative to shRAMP1 cells (top panel). GSEA enrichment score plots for selected gene sets (bottom panel). Normalized enrichment score (NES), nominal P value (statistical significance of enrichment), and false-discovery rate (FDR) are presented. B and C: RT-qPCR analysis of MAP2K1 expression in RAMP1 knockdown cells. Results are representative of at least two independent experiments performed in triplicate. Data are reported as means ± SEM. ∗P < 0.05. D: Western blot analysis of ERK1/2 activation in shControl and shRAMP1 cells. Cells were serum starved for 24 hours, induced with 10% fetal bovine serum, and harvested at 0-, 15-, 30-, and 60-minute time points. Results are representative of at least two independent experiments.