Chemokine receptor expression by decidual and peripheral NK cells. Decidual and peripheral lymphocyte suspensions were stained with fluorescence-conjugated monoclonal antibodies reactive with CD3, CD16, CD56, the indicated chemokine receptors, or with the appropriate isotype control antibodies. Initially, cells were gated by forward scatter/side scatter characteristics. CD56 and CD16 expression were each analyzed after setting a gate on CD3− cells. A: Representative histograms of chemokine receptor expression in NK cells. Isotype: filled gray curve; anti-chemokine receptor antibody: solid black curve. CD56brightCD16− dNK cells, minority CD56brightCD16− pNK cells, and majority CD56dimCD16+ pNK cells. B: Mean fluorescence intensity (MFI) ± SD of chemokine receptor expression in NK cell from three donors.