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. 2013 Sep;183(3):841–856. doi: 10.1016/j.ajpath.2013.05.029

Figure 3.

Figure 3

Separate and interactive effects of TNF-α or IL-1β or IFN-γ on IP-10 and I-TAC protein and mRNA expression in cultured first trimester decidual cells. Leukocyte-free first trimester decidual cells were primed in E2 + MPA (CON). Cultures were then switched to defined medium (DM) with the steroids and either IL-1β or TNF-α or IFN-γ or TNF-α + IFN-γ or IL-1β+ IFN-γ for 6 hours for mRNA or 24 hours for protein analysis. The mRNA levels for IP-10 (A) and I-TAC (B) were measured by RT-qPCR on RNA extracts and normalized to β-actin, a reference gene (n = 8, mean ± SEM). Protein levels of IP-10 (C) and I-TAC (D) were measured by ELISA in conditioned medium supernatants and normalized to total cell protein (n = 10, mean ± SEM). See Materials and Methods for details. *P < 0.05 versus CON; †P < 0.05 versus TNF-α alone; ‡P < 0.05 versus IL-1β alone. Western blotting (E) was carried out for IP-10 and I-TAC on 24 hours conditioned medium. Recombinant human IP-10 or I-TAC (2.5 ng per lane) are shown as positive controls (Pos) (see Materials and Methods for details).