Effects of siRNA-mediated knockdown of IFN-γR1 and IP-10, I-TAC, IFN-γR1, and IFN-γR2 mRNA expression. A–C: Gene knockdown of human IFN-γR1, or IFN-γR2, or both. Levels of β-actin are shown as a nontargeted negative control. A: Single IFN-γR1 gene knockdown. Cells were transfected with control siRNA (siCon) or siR1, and the levels of IFN-γR1 mRNAs are shown on the ordinate. B: Single IFN-γR2 gene knockdown. Cells were transfected with siCon or siR2, and the levels of IFN-γR2 mRNAs are shown on the ordinate. C: Double knockdown of IFN-γR1 and IFN-γR2. Cells were transfected with siCon, or a combination of siR1 and siR2, and the levels of IFN-γR1 and IFN-γR2 mRNAs are shown. The levels of the indicated mRNAs from siCon-transfected cells were arbitrarily set as 1. Error bars indicate mean ± SD (n = 3). D and E: Human leukocyte-free first trimester decidual cells were transfected with siRNAs specific for siR1, siR2, or siCon for 24 hours and then incubated with either 1 ng/mL TNF-α or IFN-γ or both for 6 hours (n = 1). Levels of mRNA were measured using real-time and RT-qPCR for IP-10 (D) and I-TAC (E).