Skip to main content
. 2013 Sep;183(3):841–856. doi: 10.1016/j.ajpath.2013.05.029

Figure 8.

Figure 8

Effects of IP-10 and I-TAC on CXCR3 expression by pNK cells. The lymphocyte population from healthy human donor blood was enriched by Ficoll density gradient centrifugation, followed by purification of pNK cells using NK cell isolation kits (see Materials and Methods). Enriched (91% to 95%) pNK cells were incubated with 0, 0.1, 1, 10, or 50 ng/mL IP-10 (A) or I-TAC (B) or IP-10 plus I-TAC (C) for 48 hours. After incubation, CXCR3 expression was analyzed on gated CD56+CD3− NK cells. Data are presented as means ± SEM (n = 7), with CXCR3 expression levels of cells incubated with 0 ng/mL IP-10 and I-TAC arbitrarily set as 1. The bar groups represent CD56dimCD16+ and CD56brightCD16− subsets, respectively. ∗∗P < 0.01 0 ng/mL versus 0.1, 1, 10, or 50 ng/mL IP-10 and I-TAC, by Student's t-test.