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. 2013 Sep;183(3):796–807. doi: 10.1016/j.ajpath.2013.06.008

Figure 2.

Figure 2

Omniscan-induced cells express procollagen-1, macrophage markers, iron metabolism proteins, and proangiogenic markers as shown by immunofluorescence. A: Omniscan-induced, human PBMC–derived adherent cells expressed macrophage marker-CD163 (red) as shown by immunofluorescence. Omniscan-induced cells significantly expressed CD163 (red) and procollagen-1 (green) compared with control cells (double-positive for CD163 and procollagen-1, yellow). The majority of procollagen-1–expressing cells (red) did not express CD34 (green). CD34 was expressed only weakly, and by a small number of CD163+ cells (CD34-red and CD163-green). B: Omniscan-induced cells express the iron metabolism proteins H-ferritin (green, top panel; red, middle panel), hemeoxygenase-1 (HO-1) (red), and ferroportin (green). C: Compared with control cells, Omniscan-induced cells significantly express the endothelial/pro-angiogenic markers acetylated low-density lipoprotein (AcLDL) (green), Ulex-lectin (red), Tie-2 (red), and von Willebrand factor (VWF) (green). Scale bars: 100 μm. For all immunostaining, appropriate controls included secondary antibody alone and isotype controls (data not shown).