Scheme 3. (a) Binding of 13 and 9 to Streptavidin beads, and release of GFP (S147C) 7 with EDT (1×); (b) Fluorescence spectra of GFP (S147C) 7 released from bead washes after incubation of 14 and 15 with EDT (1×) at 37 °C for 4 h.
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