Figure 1.
Binding sites between the CENP-C central region on the chimeric nucleosome mapped by methyl-based NMR. (A) Histone methyl chemical shift perturbation (CSP) upon CENP-C426-537 binding. (B) Effects on methyl groups by paramagnetic spin labels in CENP-C484-537. (C) Nucleosome structure showing representative side chains (black balls) whose methyl groups display large changes in (A) or (B), and approximate locations of CENP-C residues (arrows). E133 in H3 is used to represent L133 in H31-132-LEEGLG.