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. Author manuscript; available in PMC: 2014 Aug 19.
Published in final edited form as: Chem Res Toxicol. 2013 Aug 8;26(8):1229–1239. doi: 10.1021/tx400147c

Figure 1.

Figure 1

Methods for DNA damage detection and identification: (A) ECL screening array showing enzyme/DNA film assembled on array spots and ECL detection utilizing a dark box equipped with a CCD camera; (B) magnetic particle biocolloid reactors and 96-well plate for high-throughput LC-MS/MS detection of DNA adducts. Incubation of assembled biocolloid reactors with damage agent and subsequent DNA hydrolysis is done in a 96-well filter plate. Solutions containing hydrolyzed products of DNA and hydrolysis enzymes are transferred into a second filter plate. A third plate is used to collect filtered samples containing the nucleoside adducts for LC-MS/MS analyses.