Skip to main content
. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Mar 25;1834(7):1271–1278. doi: 10.1016/j.bbapap.2013.03.007

Figure 7. Structure of the RIIb holoenzyme traps both products in the crystal lattice.

Figure 7

The tetrameric RIIb holoenzyme is assembled as two R:C heterodimers . the dimer interface in the holoenzyme is created by the b4-b5 loop in the CNB-A domain of the R-subunit and the FDDY motif in the C-terminal tail of the C-subunit. The C-Tail is in a fully closed conformation in the tetramer even though no nucleotide is present. When MgATP was added to the crystals, the phosphate was transferred and both products, ADP and the phosphorylated RIIb subunit including two Mg2+ ions were trapped in the crystal lattice.