Fig. 3.

Comparative efficacy profiling of the 89 oncology drugs against the parental SUM149 and isogenic rSUM149 and rrSUM149 cells. Heat map profile for proliferation inhibitory effects of the NCI approved oncology set. SUM149 (A), rSUM149 (B) and rrSUM149 (C). Cells were incubated with drugs in dose response as a 10-point two-fold dilution series for 72 h and cell proliferation assessed by MTT assay as described in Materials and Methods. Each row represents dose response data for a single drug. For each concentration, percent inhibition values were calculated and data normalized to vehicle and no cell control wells. The heat map key indicates red for 100% inhibition through to green for 0% inhibition. Outlier values are shown as grey. Drug names are listed on the vertical axis and grouped for drug class as detailed in Supplemental Table 2. A representative experiment is shown. Each cell line was profiled versus the NCI Oncology set for a minimum of two independent screens. Dose response curves were generated using GraphPad Prism 5.0.