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. Author manuscript; available in PMC: 2013 Sep 5.
Published in final edited form as: Lab Invest. 2009 Oct 12;89(12):1423–1432. doi: 10.1038/labinvest.2009.113

Figure 2.

Figure 2

Optimal cell binding conditions for the CD30 aptamer. (a) Effect of MgCl2 on cell binding. Karpas 299 cells were incubated with the CD30 aptamer in PBS (pH 7.4) with varying concentrations of MgCl2 ranging from 0 to 3mM. Resultant aptamer–cell binding was detected by flow cytometry analysis. (b) Effect of CaCl2. The cell binding assay was repeated with varying concentrations of CaCl2 ranging from 0 to 3mM. (c) Effect of BSA. The cell binding assay was repeated with varying concentrations of BSA ranging from 0 to 0.5% (wt/wt). (d–f) Dosage study of the CD30 aptamer. Cultured Karpas 299 (d), SUDHL-1 (e), and Jurkat (f) cells were incubated with increasing concentrations of CD30 aptamer from 0 to 3 nM as indicated. The resultant aptamer–cell binding (%) was detected by flow cytometry analysis.